Proteins

Zinc finger-like protein (2)

RING-finger E3 ubiquitin ligase which catalyzes the formation of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Plays an important role in virulence by acting as an anti-apoptotic factor. 

Zinc finger-like protein (1)

Virulence protein 

Virulence protein

Virion protein D2

Late protein which is part of a large complex required for early virion morphogenesis. This complex participates in the formation of virosomes and the incorporation of virosomal contents into nascent immature virions. 

Virion morphogenesis protein

Lipid-bound viral membrane assembly protein that plays an essential role in immature virion (IV) to mature virion (MV) transition. Functions in both crescent-shaped viral membranes formation and its enclosure to form immature virions. In addition, participates in targeting mature virion proteins to sites of virion assembly to ensure their correct localization. 

Virion core protein p4b

Major component of the virion core that undergoes proteolytic processing during the immature virion (IV) to mature virion (MV) transition. Essential for the formation of a structurally normal core. 

Virion core protein p4a

Core protein 4a is the most abundant virion protein. Major component of the virion core that undergoes proteolytic processing during the immature virion (IV) to mature virion (MV) transition. 

Virion core protein e11

General function(s) of a protein 

Virion core protein d3

Late protein which is part of a large complex required for early virion morphogenesis. This complex participates in the formation of virosomes and the incorporation of virosomal contents into nascent immature virions. 

Viral membrane formation protein

Required for viral crescent formation early during virus morphogenesis. 

Viral core cysteine proteinase

General function(s) of a protein 

Uracil DNA glycosylase superfamily

Plays an essential role in viral replication as a component of the DNA polymerase processivity factor. Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. 

Tyrser protein phosphatase

Serine/tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the OPG144 protein. 

Type I membrane glycoprotein

Thymidylate-kinase

Thymidine kinase

Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell. 

Telomere binding protein i6-1

Telomere binding protein i1

DNA-binding protein which binds to the hairpin form of the viral telomeric sequence. Required for the production of mature virions (MV). 

Telomere binding protein

Binds to the hairpin form of the viral telomeric sequence. Might direct genome encapsidation into the virus particle. 

Sulfhydryl oxidase

Soluble interferon gamma receptor-like protein

Ser-thr kinase

Serpin 2

Serpin (3)

Serpin

Serine threonine protein kinase

Essential serine-protein kinase involved in the early stage of virion morphogenesis. 

Ser thr kinase

Schlafen 1

Nuclease that is responsible for viral evasion of host cGAS-STING innate immunity (PubMed:30728498).

Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of intracellular foreign DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses (PubMed:30728498) 

S-S bond formation pathway protein

Late protein which probably participates in disulfide bond formation by functioning as a thiol-disulfide transfer protein between membrane-associated OPG072 and OPG08. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of OPG072, OPG128 and OPG08 .

 RNA polymerase subunit RPO18

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of OPG118 and OPG133, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase. 

RNA polymerase associated transcription specificity factor rap94

RNA helicase nph-ii 2

NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs and plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for replication and propagation of viral particles. 

Rifampicin resistance protein

Ribonucleotide reductase small subunit

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. 

Ribonucleoside diphosphate reductase 2

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.automatic annotation

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Retroviral pseudoprotease-like protein

Putative monoglyceride lipase

Protein f16 (1)

Protein f15

Protein f14 (1)

Protein f11

Stimulates increases in peripheral microtubule dynamics and may increase the motility of the infected cells, contributing to cell-to-cell spread of the virus. Seems to inhibit the signaling via the GTPase RHOA and DIAPH1/mDia. 

Profilin domain protein

Poly(A) polymerase catalytic subunit (3)

Polymerase that creates the 3'-poly(A) tail of mRNA's. 

Phospholipase d like protein

Palmytilated eev membrane protein

Orthopoxvirus a26l a30l protein

Nudix domain protein

Acts with RNA polymerase to initiate transcription from late gene promoters. 

Nucleoside triphosphatase i

Nucleic acid binding protein vp8l4r

Major core structural protein. 

Ntpase (1)

Nlpc p60 superfamily protein

Nfkb inhibitor

Myristylated protein (2)

Myristylated protein (2)

Myristylated protein

Myristoylated protein e7

MV membrane efc component

Mutt motif protein

Acts with RNA polymerase to initiate transcription from late gene promoters. 

mRNA capping enzyme small subunit

Acts with RNA polymerase to initiate transcription from late gene promoters. 

mRNA capping enzyme large subunit

MPXV gp150

Plays a role in the inhibition of host protein synthesis. Specifically, inhibits the initiation of cap-dependent and cap-independent translation. In turn, affects the outcome of infection by decreasing recruitment of inflammatory leukocytes and reducing the memory CD8+ T-cell response. 

MHC class ii antigen presentation inhibitor

Metalloendopeptidase

Probably involved in maturation of some viral proteins by processing them preferentially at Ala-Gly-|-Ser/Thr/Lys motifs. Does not seem to be responsible for the cleavage of major core proteins. 

Membrane protein e8

Membrane protein cl5

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation. 

M137r

Late transcription factor vltf-4 (1)

Late transcription factor vltf-3(1)

Acts with RNA polymerase to initiate transcription from late gene promoters .

Late transcription factor vltf 2(2)

Acts with RNA polymerase to initiate transcription from late gene promoters. 

Late transcription factor vltf 1

Associates with RNA polymerase to initiate transcription from late gene promoters. 

Late transcription elongation factor

Involved in postreplicative transcription elongation on intermediate and late genes. 

Late protein h7

Kelch-like protein (2)

Kelch like-protein (1)

Intracellular viral protein

Internal virion l3 fp4 protein

Might be required to be present in the virion for transcription of early genes after primo infection. 

Intermediate transcription factor vitf-3 (2)

Acts with RNA polymerase to initiate transcription from intermediate gene promoters. 

Intermediate transcription factor vitf-3(1)

Acts with RNA polymerase to initiate transcription from intermediate gene promoters. 

Interleukin 18 binding protein

IMV surface fusion protein

IMV membrane protein p21

IMV membrane protein l1r (2)

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation. 

IMV membrane protein l1r

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation. 

IMV membrane protein j1

IMV membrane protein i5

Envelope protein. 

IMV membrane protein i2

Late protein which probably plays a role in virus entry into the host cell. 

IMV membrane protein e6

Plays an essential role for maintaining proper localization of the seven-protein complex and the viroplasm during assembly. 

IMV membrane protein a30

Required for the association between the dense viroplasm and the viral membranes to form the mature virion (MV). 

IMV membrane protein a21

IMV membrane protein a14

Envelope protein which is a major component of the mature virion (MV) membrane. Essential for membrane biogenesis. Is required, together with OPG144, to form bona fide crescents, which can progress to form the immature virion (IV) membrane. OPG140 and OPG144 form a lattice that is stabilized by disulfide bonds and serves as an anchor within the viral membrane to which several other proteins important in virion structure and morphogenesis attach. 

IMV membrane protein a13l

Essential for the encapsidation of DNA into immature virions (IV) and the subsequent maturation of IV into mature virions (MV). 

IMV membrane protein a9

Envelope protein. Required for an early step in virion morphogenesis. 

IMV heparin binding surface protein

IL 1 receptor homolog

IFN alphabeta receptor-like secreted glycoprotein

IEV transmembrane phosphoprotein

IEV morphogenesis protein(2)

IEV morphogenesis protein

Hypothetical protein mpxv gp145

Hydroxysteroid dehydrogenase

Catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. During viral infection, steroid production contributes to virulence by inhibiting the host inflammatory response.

Host range protein (1)

Holliday junction resolvase (1)

Plays a role in DNA replication by cleaving viral DNA concatamers to yield unit-length viral genomes. The concatamer junctions contain inverted repeat sequences that can be extruded as cruciforms, yielding Holliday junctions that A22 protein cleaves. 

Hemagglutinin

Glutaredoxin 2 (1)

Glutaredoxin necessary for virion morphogenesis and virus replication. Functions as a thiol-disulfide transfer protein between membrane-associated OPG128 and substrates OPG095 or OPG053. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of OPG072, OPG128 and OPG088. Exhibit thioltransferase and dehydroascorbate reductase activities in vitro. 

Glutaredoxin 1 (1)

Displays thioltransferase and dehydroascorbate reductase activities. 

FEN1 like nuclease

Putative nuclease that seems to be required for double-strand break repair, homologous recombination, and production of full-length viral genomic DNA. 

Envelope protein a28 homolog (1)

Envelope protein required for virus entry into host cell and for cell-cell fusion (syncytium formation). 

Entry fusion complex component (1)

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation. 

Entry fusion complex essential component (1)

Envelope protein part of the entry-fusion complex responsible for the virus membrane fusion with host cell membrane during virus entry. Also plays a role in cell-cell fusion (syncytium formation). 

EGF like domain protein (1)

EEV type i membrane glycoprotein (1)

EEV maturation protein

EEV glycoprotein (2)

EEV glycoprotein (1)

Early transcription factor 82 kda subunit (1)

Early transcription factor 70 kda subunit

Acts with RNA polymerase to initiate transcription from early gene promoters. Is recruited by the RPO-associated protein of 94 kDa RAP94/OPG109 to form the early transcription complex, which also contains the core RNA polymerase. ETF heterodimer binds to early gene promoters. 

Dutpase

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. 

Duf1029 domain protein

Double stranded rna binding protein

DNA topoisomerase   type i

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. 

DNA polymerase processivity factor

DNA polymerase 2

DNA ligase 2

DNA helicase

DNA helicase which seems to act as a postreplicative transcription termination factor. Involved in ATP-dependent release of nascent RNA. Forms a stable complex with single-stranded DNA, and to a lesser extent RNA. 

DNA directed RNA polymerase subunit

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of OPG118 and OPG133, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase. 

DNA directed RNA polymerase 35 kda subunit

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. 

DNA directed RNA polymerase 30 kda polypeptide

DNA directed RNA polymerase 19 kda subunit

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of OPG118 and OPG133, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase. 

DNA directed RNA polymerase 7 kda subunit

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of OPG118 and OPG134, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase. 

DNA dependent RNA polymerase subunit rpo147

DNA dependent RNA polymerase subunit rpo132

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase (By similarity). 

DNA binding phosphoprotein 2

Plays an essential role in viral DNA replication. Binds to ssDNA with high affinity and localizes to cytoplasmic factories where nascent viral genomes accumulate. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. 

DNA binding phosphoprotein 1

Plays an essential role in virion assembly and morphogenesis. Also plays a role in the inhibition of host immune response by dysregulating mTOR. Sequesters host RICTOR and RPTOR, thereby disrupting mTORC1 and mTORC2 crosstalk. In turn, blocks the host antiviral response in part through mTOR-dependent degradation of cGAS, the primary poxvirus sensor. 

Cytoplasmic protein

Cytochrome c oxidase

crm b secreted tnf alpha receptor like protein (2)

crm b secreted tnf alpha receptor like protein

Crescent membrane and immature virion formation protein

Early protein involved in virion morphogenesis. Participates in the formation and elongation of crescent-shaped membrane precursors of immature virions in cytoplasmic factories. 

cpxv173 protein

cpxv166_protein

cpxv054 protein

cpxv053_protein

Core protein a15

Late protein which is a part of a large complex required for early virion morphogenesis. This complex participates in the formation of virosomes and the incorporation of virosomal contents into nascent immature virions. Required for the stability and kinase activity of OPG054. 

Copper-zinc superoxide dismutase

Virion protein with no enzymatic activity. 

Complement control protein ccp

Chemokine binding protein (1)

Chemokine binding protein (2)

Chemokine binding protein

cd47 like protein

Promotes, when overexpressed, the influx of extracellular Ca2+, leading to membrane permeability and host cell necrosis. 

caspase 9 inhibitor

Carbonic anhydrase

Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell. 

Cap specific mrna

Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase OPG063 that creates mRNA's poly(A) tail. In the presence of OPG102, OPG063 does not dissociate from the RNA allowing tail elongation to around 250 adenylates. 

C4lc10l like family protein

Bcl 2 like protein 8

Bcl 2 like protein 7

Bcl 2 like protein 6

Bcl 2 like protein 5

Bcl 2 like protein 4

Bcl 2 like protein 3

Bcl 2 like protein 2

Bcl_2_like_protein

B22r family protein

Brix domain protein

Atpase_a32

Participates in viral DNA packaging and virion morphogenesis. 

Assembly protein G7

Late protein which is a part of a large complex required for early virion morphogenesis. This complex participates in the formation of virosomes 

Ankyrin-repeat protein (44)

MPXV ankyrin-like proteins are known to inhibit host antiviral signaling and inflammatory responses by disrupting activation of NF-κB22

Ankyrin-repeat protein 39 (2)

Ankyrin-repeat protein (39)

plays a significant role in the virus's ability to evade the host's immune response.

Ankyrin-repeat protein 25_2

plays a significant role in the virus's ability to evade the host's immune response.

Ankyrin-repeat protein 25 (2)

plays a significant role in the virus's ability to evade the host's immune response.

Ankyrin-repeat protein (25)

plays a significant role in the virus's ability to evade the host's immune response.

Ankyrin-repeat protein (14)

MPXV ankyrin-like proteins are known to inhibit host antiviral signaling and inflammatory responses by disrupting activation of NF-κB22

Ankyrin-repeat protein (2)

Plays a role in the inhibition of host immune repsonse by counteracting the action of interferons on early events in the viral replication cycle.

Ankyrin-like protein (46)

inhibit host antiviral signaling and inflammatory responses by disrupting activation of NF-κB22

Ankyrin-like protein (3)

Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin

Ankyrin-like protein (1)

 ankyrin-like; ANK-containing protein; apoptosis inhibitor; start of ORF is missing approx. 31 AA compared to CPXVs; similar to VACV-WR O1L and VACV-Cop M1L

A32.5L

Viral membrane assembly proteins (VMAP)

A12 Protein

Component of the virion core that undergoes proteolytic processing during the immature virion (IV) to mature virion (MV) transition. Essential for the formation of a structurally normal core

A5L protein

immunodominant virion core protein needed for the progression of IV to infectious IMV